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   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>f8c0b295-a1a0-465b-ab27-ca8772f1e525</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="8:4:9">2025.5.13</Created>
<LastModified UTC="6:26:54">2025.10.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  e<StrandColors><TopStrand><ColorRange range="0..2885" colors="black"/><ColorRange range="2886..3606" colors="green"/><ColorRange range="3607..3832" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2885" colors="black"/><ColorRange range="2886..3606" colors="green"/><ColorRange range="3607..3832" colors="black"/></BottomStrand></StrandColors>  %     Û          ìES¥uîJ®®Ž©êž¾–{RuªXzªz§µ%äôgªz¥¡%Z¯å'¢z·¡%Z¨Iê§~ák%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öaÑ•'¡%ªzxž”šj ªzž„�iUµž©êž–�ª©UIê§¾�jš�-U'¦ª�zFåãEÉÅüs'¹o|,÷Ø!5A—`öÛPJØÎµÝ�¼…ÃÜJÄl@Ä@óþqGÏS…}@ƒÿ�ë>Ûâÿ´­‰Ã½Cç!Mÿ<vÿzâúÿ:Ô€LÈazÄ0È!òÐ{ïãAÃ¶çP…8Ì7×¯ûÇòXÑE`8>ûÄÁ‰ÿpÏá@ÄmMáN0÷œs„-Gß�CÔSò4ãðÏ�r×mJmû÷\Aç}²Òqs<@óÞ÷Ã�Äâu$0óäm–ÓÙ÷j$B©èêU_¿wïþá]•acZ(¢—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J �ø        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>f8c0b295-a1a0-465b-ab27-ca8772f1e525</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="8:4:9">2025.5.13</Created>
<LastModified UTC="6:25:45">2025.10.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  e<StrandColors><TopStrand><ColorRange range="0..2885" colors="black"/><ColorRange range="2886..3615" colors="green"/><ColorRange range="3616..3841" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2885" colors="black"/><ColorRange range="2886..3615" colors="green"/><ColorRange range="3616..3841" colors="black"/></BottomStrand></StrandColors>  N     Û          ìES¥uîJ®®Ž©êž¾–{RuªXzªz§µ%äôgªz¥¡%Z¯å'¢z·¡%Z¨Iê§~ák%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öaÑ•'¡%ªzxž”šj ªzž„�iUµž©êž–�ª©UIê§¾�jš�-U'¦ª�zFåãEÉÅüs'¹o|,÷Ø!5A—`öÛPJØÎµÝ�¼…ÃÜJÄl@Ä@óþqGÏS…}@ƒÿ�ë>Ûâÿ´­‰Ã½Cç!Mÿ<vÿzâúÿ:Ô€LÈazÄ0È!òÐ{ïãAÃ¶çP…8Ì7×¯ûÇòXÑE`8>ûÄÁ‰ÿpÏá@ÄmMáN0÷œs„-Gß�CÔSò4ãðÏ�r×mJmû÷\Aç}²Òqs<@óÞ÷Ã�Äâu$L<Ç¹e´ö}Ú‰ªz:•WïÝûÿ…øWeXXÖŠ(¥è©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ$>        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ü<?xml version="1.0"?><Features recycledIDs="0" nextValidID="10"><Feature recentID="8" name="mCherry" directionality="1" translationMW="26722.72" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2906-3616" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;monomeric derivative of DsRed fluorescent protein (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/15558047&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/15558047&quot;&gt;Shaner et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2178" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="1387-2178" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="2179-2247" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="628-1216" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2248-2352" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3739-3820" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="274-280" color="#ffffff" type="standard"/><Segment range="281-298" color="#ffffff" type="standard"/><Segment name="-35" range="299-304" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="319-340" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="226-242" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="250-266" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   ¯<?xml version="1.0"?><Primers recycledIDs="3,2,1,0,4" nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>f8c0b295-a1a0-465b-ab27-ca8772f1e525</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="8:4:9">2025.5.13</Created>
<LastModified UTC="6:21:54">2025.10.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<Created UTC="8:4:9">2025.5.13</Created>
<LastModified UTC="6:20:35">2025.10.23</LastModified>
<SequenceClass>UNA</SequenceClass>
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<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pTol2-MCS-TagBFP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="8:4:9">2025.5.13</Created>
<LastModified UTC="13:26:45">2026.3.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO ÿþ              E              ÿÿÿÿYô? P                                                                                                                                                             e<StrandColors><TopStrand><ColorRange range="0..2885" colors="black"/><ColorRange range="2886..3658" colors="green"/><ColorRange range="3659..3833" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2885" colors="black"/><ColorRange range="2886..3658" colors="green"/><ColorRange range="3659..3833" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
